Biofunctionalized Devices—On Chip Signaling and 'Rewiring' Bacterial Cell-to-Cell Communication
Prof. Reza Ghodssi
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| Dr. Reza Ghodssi |
The goal of the research, titled "Biofunctionalized Devices—On Chip Signaling and 'Rewiring' Bacterial Cell-Cell Communication," is to demonstrate signal translation by employing device-based electrical signals to guide the assembly of biosynthetic pathways, cell-based sensors, and cell-based actuators within a microelectromechnical system (MEMS); and to use on-board electrical, magnetic, mechanical, and optical systems to feedback and guide the cell-based system towards user-specified outcomes. The focus of this project is the cell to cell communication system mediated by bacterial signaling autoinducers in a process known as quorum sensing. The PIs have created a computational model that captures the dynamics of quorum signal generation, receptor driven recognition, and uptake. This model, based on biochemical and biophysical processes, will help the conceptual design of subsystem synthons assembled architectures that guide heterologous protein synthesis in response to specific biomolecular cues. Cells will be signaled to initiate biofilm formation and maturation. The MEMS environment will enable, for the first time, an experimental platform for the design, construction, and testing of this cell-based signal transduction process. Moreover, this MEMS environment will detect cell function and, by guiding signaling pathways, change cell phenotype in a controlled and directed manner.
The principal investigator for the project is Professor William Bentley, chair of the University of Maryland's Fischell Department of Bioengineering. Professor Reza Ghodssi (ECE/ISR) and Maryland NanoCenter Director and Professor Gary Rubloff (MSE/ECE/ISR) are also part of the research team. The investigative team also includes Gregory Payne, director of the Center for Biosystems Research at the University of Maryland Biotechnology Institute. Ghodssi is the principal investigator for the portion of the grant related to the Electrical and Computer Engineering (ECE) Department and the Institute for Systems Research (ISR).
The PIs will enlist guidance and support from industry, which may spawn new efforts in device fabrication, embedded sensor systems, bacterial pathogenicity, biofilm formation, genetic regulation and signal transduction. Developments are envisioned that impact fields of medicine (drug discovery, synthesis, and delivery), communications (biofunctionalized microfabricated devices), and security (smart sensors).
A four-year grant worth $1,968,984 from the National Science Foundation (NSF) Emerging Frontiers in Research and Innovation-Cellular and Biomolecular Engineering (EFRI-CBE) will support the research. EFRI is NSF's newest and most competitive grant; a total of only 12 were awarded.
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